High-affinity binding of interferon-gamma to a basement membrane complex (matrigel).

Histones Have High Affinity for the Glomerular Basement Membrane

Histones are a set of highly cationic proteins essentially involved in the binding andcompaction of DNA in the cell nucleus chromatin (1-3) . In mammals, five subclasses of histones exist: H1 (f1), H2a (f2a2), H2b (f2b), H3 (f3), and H4 (f2a1) (4,5), whereby four histones form an octameric core protein aggregate (H2a, H2b, H3, H4) around which a 145-bp DNA molecule is wrapped, forming the core ...

Interferon gamma and lipopolysaccharide promote macrophage adherence to basement membrane glycoproteins

The ability of thioglycollate (TG)-elicited peritoneal macrophages, a population of recently recruited monocytes, to adhere to the basement membrane glycoproteins laminin and type IV collagen is not a constitutive function of these cells. Adherence can be induced, however, by treatment with IFN-gamma and LPS. In general, IFN-gamma is more potent than LPS in promoting this adherence. Maximal adh...

High affinity binding of 125I-angiotensin II to rat glomerular basement membranes.

125I-angiotensin II (AII) specifically bound to rat glomerular basement membrane (GBM). The kinetics of binding were similar to those obtained with the total glomeruli. The apparent dissociation constant was close to 50 pM with both preparations. The number of sites related to the amount of protein was two times greater with GBM than with total glomeruli. Since the amount of GBM protein extract...

Matrigel Model Basement Membrane a Effects on Jurkat T Cell Migration Through Lysophosphatidic Acid Receptor-Selective

Histones have high affinity for the glomerular basement membrane. Relevance for immune complex formation in lupus nephritis

An effort has been made to integrate insights on charge-based interactions in immune complex glomerulonephritis with nuclear antigen involvement in lupus nephritis. Attention was focussed on the histones, a group of highly cationic nuclear constituents, which could be expected to bind to fixed anionic sites present in the glomerular basement membrane (GBM). We demonstrated that all histone subf...